Fig. 3

Blockade of TUFT1 inhibited TGF-β1-induced fibrosis in vitro. a Interference efficiency of TUFT1 specified siRNA in the A549 cells detected by Western blotting. b The microfilaments morphology of the A549 cells was revealed by the phalloidin staining in si-TUFT1 groups and control, scale bars: 10 µm. F-actin was quantified by the percentage of the cells with the actin cores. c, d The wound healing progression of A549 cells in si-TUFT1 groups and control. e, f The migration ability of A549 cells in si-TUFT1 groups and control, scale bars: 1 mm. g The microfilaments morphology of the MRC-5 cells was revealed by the phalloidin staining in si-TUFT1 groups and control, scale bars: 100 µm. F-actin was quantified by the percentage of the cells with the actin cores. h The expression of FN, collagen I, and α-SMA proteins were determined by Western blot after the silence of TUFT1 in TGF-β1-induced MRC-5 cells. i, j The wound healing progression of MRC-5 cells in si-TUFT1 groups and control. k, l The activation of fibroblasts was examined based on fibroblast contraction in 3D collagen matrices (n = 3 independent experiments). ImageJ software was used to measure the area of gels. *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001