Fig. 3

The effect of anti-CD147 in OVA-induced vascular remodeling in asthmatic mice

Mice asthma models were induced by OVA, and treated with anti-CD147 or Isotype antibody. Mice treated only with PBS were used as controls. (A) Representative immunohistochemical images of CD31 protein in lung sections, its positive signal appears pale yellow or yellow-brown and nuclei appear blue. Magnification ×400, scale bar = 50 μm. (B) Quantification of vessels area (n = 6). (C) Representative immunohistofluorescence images of α-SMA protein in lung sections, its positive signal appears green. Magnification ×200, scale bar = 100 μm. (D) Quantification of the thickness of vascular smooth muscle around the bronchioles (n = 6). (E) Representative western blotting images of VEGF, α-SMA and quantification of their protein expression normalized to GAPDH (n = 3). Data were presented as mean ± SD, and analyzed by one-way ANOVA with Bonferroni correction. *P < 0.05, vs. control; ^#P < 0.05, vs. OVA