Table 1 Changes in interleukin-5 (IL-5), nuclear factor-κB (NF-kB) and histone deacetylase-2 (HDAC-2) levels (pg/ml) from pretreatment values induced by fluticasone furoate (FF) and umeclidinium (UME) at different concentrations, alone or in combination, in non-sensitised and sensitised human bronchial epithelial cells (HBEpCs).
Non-sensitized HEBpCs | Sensitized HEBpCs | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Pretreatment | FF 150 nM | FF 1.5 µM | UME 128 nM | UME 1.28 µM | FF 150 + UME 128 nM | FF1.5 + UME 1.28 µM | Pretreatment | FF 150 nM | FF 1.5 µM | UME 128 nM | UME 1.28 µM | FF 150 + UME 128 nM | FF1.5 + UME 1.28 µM | |
IL-5 | 176.1 ± 23.7 | -9.77 ± 8.5 | -9.52 ± 3.1 | -9,52 ± 9.6 | -13.63 ± 4.7 | -15.33 ± 7.5 | -10.4 ± 3.3 | 283.9 ± 9.4 | -77.6 ± 46.5 | -98.3 ± 29.2 * | + 37.9 ± 23.2 | -41.8 ± 55.0 | -72.4 ± 18.9 ** | -154.3 ± 22.5 *** |
NF-kB | 1.37 ± 0.06 | -0.02 ± 0.04 | -0.05 ± 0.03 | -0.05 ± 0.03 | -0.03 ± 0.03 | -0.06 ± 0.03 | -0.08 ± 0.02 | 3.44 ± 0.18 | -0.94 ± 0.21 ** | -0.67 ± 0.38 | -0.53 ± 0.24 | -0.95 ± 0.31 * | -1.05 ± 0.28 * | -1.22 ± 0.40 * |
HDAC-2 | 1.87 ± 0.51 | + 0.17 ± 0.04 | + 0.18 ± 0.06 | + 0.02 ± 0.09 | + 0.10 ± 0.03 | + 0.11 ± 0.09 | + 0.19 ± 0.09 | 0.48 ± 0.22 | + 1.49 ± 0.25 ** | + 1.36 ± 0.40 * | + 0.24 ± 0.20 | -0.14 ± 0.20 | + 1.79 ± 0.21 *** | + 2.31 ± 0.63 * |