Table 2 Changes in acetylcholine (ACh) and choline acetyltransferase (ChAT) levels (pg/ml) from pretreatment values induced by fluticasone furoate (FF) and umeclidinium (UME) at different concentrations, alone or in combination, in non-sensitised and sensitised human bronchial epithelial cells (HBEpCs)
Non-sensitized HEBpCs | Sensitized HEBpCs | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Pretreatment | FF 150 nM | FF 1.5 µM | UME 128 nM | UME 1.28 µM | FF 150 + UME 128 nM | FF1.5 + UME 1.28 µM | Pretreatment | FF 150 nM | FF 1.5 µM | UME 128 nM | UME 1.28 µM | FF 150 + UME 128 nM | FF1.5 + UME 1.28 µM | |
ACh | 8.90 ± 0.91 | + 0.12 ± 0.70 | + 0.59 ± 0.66 | + 0.96 ± 0.84 | -0.56 0.41± | + 0.75 ± 0.55 | + 0.57 ± 0.93 | 29.25 ± 3.30 | -15.52 ± 3.46 ** | -19.41 ± 3.47 ** | -17.99 ± 3.34 ** | -20.98 ± 3.31 *** | -16.82 ± 3.39 ** | -20.50 ± 3.44 *** |
ChAT | 2.09 ± 0.25 | + 0.05 ± 0.21 | -0.03 ± 0.13 | + 1.40 ± 0.33 | -0.02 ± 0.18 | -0.01 ± 0.08 | -0.13 ± 0.10 | 3.76 ± 1.00 | -1.12 ± 1.02 | -1.36 ± 1.01 | -0.89 ± 1.00 | -1.55 ± 1.00 | -0.84 ± 1.06 | -1.57 ± 1.00 |