Fig. 4
From: FGF18 alleviates sepsis-induced acute lung injury by inhibiting the NF-κB pathway
FGF18 deletion exacerbates LPS-induced lung injury. (A) FGF18+/+ and FGF18+/− mice were subjected to western blotting analysis. The expression of FGF18 was detected. (n = 5 per group). (B) Immunofluorescent staining of FGF18 (red) and DAPI (blue) in FGF18+/+ and FGF18+/− mice were detected. (n = 5 per group, Scale bar = 150 μm). (C) HE staining in lung tissues of FGF18+/+ and FGF18+/− mice after PBS or LPS injection for 12 h (n = 5 per group). (D) Western blotting was performed and quantitatively analyzed to determine the protein levels of VCAM-1, ICAM-1, IL-6, and TNF-α in the lungs from FGF18+/+ and FGF18+/− mice in the presence of LPS (n = 5 per group). (E) Immunofluorescent staining of ICAM-1/VCAM-1 (red) and DAPI (blue) in FGF18+/+ and FGF18+/− mice were detected. (n = 5 per group, Scale bar = 150 μm)