Fig. 2

RAGE is essential for CS-induced COPD progression. A Flow chart of siRNA administration in chronic obstructive pulmonary disease mice for modeling in vivo; B, C WB detection of RAGE protein levels in the lung tissue of each group of mice (n = 3); D rt-PCR was used to detect the mRNA level of Ager in the lung tissue of mice in each group (n = 6–8); E Representative images of H&E staining in mouse lung tissue; F MLI of mouse lung tissue (n = 6–8); G–I Mouse lung function indicators FEV_0.1/FVC, IC and Rn (n = 6–8); J Pearson correlation analysis between the MLI of mouse lung tissue and the level of Ager mRNA (n = 25); K Pearson correlation analysis of FEV_0.1/FVC, IC, and Rn with Ager mRNA levels in mice (n = 25); L, M statistics of the total number of inflammatory cells and neutrophils in mouse alveolar lavage fluid (BALF) (n = 6–8); N, O rt-PCR was used to detect the mRNA levels of IL-1β and TNF-α in the lung tissues of mice in each group (n = 6–8); P, Q ELISA detection of IL-6 and IL-8 protein levels in mouse lung tissue (n = 6–8); R–T flow cytometry was used to detect the polarization of macrophage M1 and M2 in mouse lung tissue; U, V flow cytometry detection of neutrophil infiltration in mouse lung tissue (n = 4–6). The experimental results were independently repeated three or more times, and Data are least squares means ± standard errors. *P < 0.05, **P < 0.01, ***p < 0.001