Fig. 3

Down-regulated miR-23a-5p can target and decrease RAGE in COPD mice. A Differentially expressed microRNAs targeting RAGE were screened through bulk RNAseq analysis and website prediction; B Pearson correlation analysis between miR-23a-5p mRNA level and FEV₁/FVC of subjects (n = 28); C Pearson correlation analysis between miR-23a-5p mRNA level and FEV₁ pred (%) of subjects (n = 28); D schematic diagram of binding sites between miR-23a-5p and AGER; E double luciferase report experiment to verify the binding of miR-23a-5p with AGER (n = 3); F, G fluorescence in situ hybridization (FISH) assay to detect the expression of miR-23a-5p in mouse lung tissue and analyze the average fluorescence intensity (MFI) (n = 3); H rt-PCR was used to detect mRNA levels of mir-23a-5p in the lung tissue of control and COPD mice (n = 4); I Model construction flowchart; J, K Western Blotting detection of RAGE protein levels in the lung tissue of mice in each group (n = 3); L–N fluorescence in situ hybridization (FISH) was used to detect the expression of miR-23a-5p and RAGE in mouse lung tissue and analyze the average fluorescence intensity (MFI) (n = 3); O, P Western Blotting detection of RAGE protein levels in 16-HBE cells transfected with miR-23a-5p mimics; Q-R: detection of RAGE protein levels in 16-HBE cells transfected with miR-23a-5p inhibitor. The experimental results were independently repeated three or more times, and Data are least squares means ± standard errors. *P < 0.05, **P < 0.01, ***p < 0.001