Fig. 4
MiR-23a-5p reduced emphysema and airway inflammation in COPD mice, leading to improved lung function. A Representative images of the H&E stain in the lung tissue of each group of mice; B MLI of mouse lung tissue (n = 6–8); C–F The levels of FEV0.1/FVC, IC, tissue elasticity (H) and Rn in different groups (n = 6–8); G, H ELISA detection of IL-6 and IL-8 protein levels in the lung tissue of mice in different groups (n = 6–8); I, J ELISA detection of IL-6 and IL-8 protein levels in BALF of different mice (n = 6–8); K–M rt-PCR was used to detect the mRNA levels of IL-1β and TNF-α in HBE cells transfected with mimics; N, O ELISA was used to detect the secretion levels of IL-6 and IL-8 in 16-HBE cells transfected with mimics. The experimental results were independently repeated three or more times, and Data are least squares means ± standard errors. *P < 0.05, **P < 0.01, ***p < 0.001