Fig. 1

Curdlan mainly enhances pulmonary neutrophil inflammation in asthmatic mice. C57BL/6 mice were randomly divided into 4 groups, PBS, HDM, HDM + Curdlan and HDM + Laminarin group. Mice received either Curdlan (20 μg Curdlan in 50 μl PBS) or Laminarin (5 mg/kg, 100 μl) prior to HDM at days 0, 2, 4, 11 to 14. A The lung tissue of each group was stained with H&E and PAS (magnification: 25x). B, C Histopathological inflammatory score were assessed based on H&E staining and PAS staining. D Levels of HDM specific IgG1 (sIgG1) in BALF were quantitated using a mouse specific ELISA kit. E–I The counts of total cells and neutrophils, eosinophils, macrophages and lymphocytes in BALF of each mice group were detected by flow cytometry. J, K Detection of IL-4 and IL-5 cytokines in BALF of mice in each group by ELISA kit. L-M. mRNA expression of IL-6 and IL-17a in the lungs of mice measured using qRT-PCR (n = 6–8) (*P < 0.05; **P < 0.01;***P < 0.005; ****P < 0.001, one way ANOVA, error bars represent mean ± SEM)