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Fig. 2 | Respiratory Research

Fig. 2

From: Early-life house dust mite aeroallergen exposure augments cigarette smoke-induced myeloid inflammation and emphysema in mice

Fig. 2

Chronic house dust mite (HDM) and cigarette smoke (CS) exposure induced airway inflammation as determined by (A) total bronchoalveolar lavage fluid (BALF) cells. Total (B) BAL macrophages, (C) neutrophils, (D) eosinophils and (E) lymphocytes were also increased, as measured by differential counts. Flow cytometry was performed on lung tissue to track myeloid cell populations with representative plots shown in (F). Lung neutrophils (G), interstitial macrophages (IM) (H), alveolar macrophages (AM) (I) and eosinophils (J) were analysed and presented as percentages over CD45 positive cells. For each biological group, n = 6. *p < 0.05; **p < 0.01; ***p < 0.001, ****p < 0.0001 2-way ANOVA with multiple comparisons across all groups and Tukey’s post-hoc test

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