Fig. 2

NR2F2 overexpression mitigated the senescence of lung epithelial cells induced by bleomycin. (A-C) SA-β-Gal staining was performed to detect the senescence of A549 (A), MLE-12 (B), and BEAS2B (C) cells treated with 0.02 U/ml bleomycin for an additional 72 h after transfection with control or NR2F2 overexpression plasmid for 48 h. (D-F) The protein expression levels of NR2F2, p21, and p16 in A549 (D), MLE-12 (E), and BEAS2B (F) cells treated with 0.02 U/ml bleomycin for an additional 72 h after transfection with control or NR2F2 overexpression plasmid for 48 h were measured using WB. (G-I) The mRNA expression levels of CDKN1A (G), CDKN2A (H), and GLB1 (I) in A549, MLE-12, and BEAS2B cells treated with 0.02 U/ml bleomycin for an additional 72 h after transfection with control or NR2F2 overexpression plasmid for 48 h were quantified using qRT-PCR. (J-K) Representative immunofluorescence staining of p21 expression in A549 (J) and BEAS2B (K) cells transfected with control or NR2F2 overexpression plasmid for 48 h, prior to challenge with bleomycin for an additional 72 h (0.02 U/ml). Data are shown as the mean ± SD. *P<0.05, **P<0.01, ***P<0.001, ****P<0.0001