Fig. 3
From: NR2F2 alleviates pulmonary fibrosis by inhibition of epithelial cell senescence
Downregulation of NR2F2 expression induced lung epithelial cell senescence. (A-C) SA-β-Gal staining was performed to assess the senescence status of A549 (A), MLE-12 (B), and BEAS2B (C) cells stably expressing control and NR2F2 knockdown plasmids. (D-F) The protein expression levels of NR2F2, p21, and p16 in A549 (D), MLE-12 (E), and BEAS2B (F) cells stably expressing control and NR2F2 knockdown plasmids were measured using WB. (G-I) The mRNA expression levels of CDKN1A, CDKN2A, and GLB1 in A549 (G), MLE-12 (H), and BEAS2B (I) cells stably expressing control and NR2F2 knockdown plasmids were quantified using qRT-PCR. (J-K) Representative immunofluorescence staining of p21 expression in A549 (J) and BEAS2B (K) cells stably expressing control and NR2F2 knockdown plasmids. Data are shown as the mean ± SD. *P<0.05, **P<0.01, ***P<0.001, ****P<0.0001