Fig. 7
From: NR2F2 alleviates pulmonary fibrosis by inhibition of epithelial cell senescence
Nr2f2 overexpression ameliorated bleomycin-induced cell senescence and inflammatory response in mouse lungs. (A) Representative images of SA-β-Gal staining in lung tissues from mice of AAV2/9 or AAV2/9-Nr2f2 infected mice treated with either saline or bleomycin. (B, C) qRT-PCR and WB analysis the changes in gene (B) and protein (C) expression of p21 and p16 in lung tissues of AAV2/9 or AAV2/9-Nr2f2 infected mice treated with either saline or bleomycin. (D) Immunofluorescence analysis for SP-C (pink) and P21 (green) in lung tissues of AAV2/9 or AAV2/9-Nr2f2 infected mice treated with either saline or bleomycin. (E) Lung/body weight ratios were calculated in AAV2/9 or AAV2/9-Nr2f2 infected mice treated with either saline or bleomycin. (F-H) Quantification of the total number of inflammatory cells (F), total protein (G), and Il-1β content (H) in BALF of mice infected with AAV2/9 or AAV2/9-Nr2f2 and treated with either saline or bleomycin. (I) qRT-PCR analysis the mRNA expression of Il-1β in lung tissues of AAV2/9 or AAV2/9-Nr2f2 infected mice treated with either saline or bleomycin. Data are shown as the mean ± SD. *P<0.05, **P<0.01, ***P<0.001, ****P<0.0001