Fig. 3
From: A novel quinoline with airway relaxant effects and anti-inflammatory properties
Immune cell recruitment and lung histology. A Total cell, (B) neutrophil, (C) inflammatory macrophage and (D) eosinophil concentration in BALF of Veh/PBS (n = 8), RCD405/PBS (n = 8), Veh/OVA (n = 8), RCD405/OVA (n = 7) and BUD/OVA (n = 7) treated mice measured by flow cytometry. E Representative Giemsa-Wright stained cytospins of BALF samples from Veh/PBS, RCD405/PBS, Veh/OVA and RCD405/OVA. F Differential counts of total cells, eosinophils, macrophages, and neutrophils in Giemsa-Wright stained cytospins from BALF samples. G H&E and (I) AB/PAS staining on 4 μm lung tissue sections from mice with the following treatments: Veh/PBS (n = 9), RCD405/PBS (n = 10), Veh/OVA (n = 9) and RCD405/OVA (n = 8). H Areas (μm2) of inflammatory cell lesions (black arrows) were measured with ImageScope software. J Mucus production was analyzed by calculating the PAS % of positive pixels in the bronchi with ImageScope software. Statistical comparisons were performed using one-way ANOVA followed by Šídák's multiple comparisons test. (ns: not significant, (*P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001 and ****P ≤ 0.0001)