Fig. 5

Differentially expressed genes and pathways in alveolar macrophages of ACO. A, A two-dimensional scatter plot of trajectory estimation of all cells from monocytes/macrophages. B, Distribution of cell clusters identified by Leiden matching trajectory analysis. C, Distribution of cells strata by groups matching trajectory analysis. D, Relative expression of lineage markers commonly used for monocytes/macrophages. E, Top-ranking high expressed marker genes across 7 cell clusters identified by FindMarkers. F, Difference of AUCell KEGG enrichment scores between Leiden cluster 1 and other clusters. G, Difference in SenMayo senescence score between ACO and control group. H-I, Relative expression of senescence markers CDKN1A (p21, H) and CDKN2A (p16, I) in ACO and control group. J, Heatmap representing the differential expression of genes linked to oxidoreductases, cytokines, and growth factors between ACO and control group. K, Correlations between CDKN1A expression and differentially expressed genes in Leiden cluster 1 and lineage markers for monocytes/macrophages. Wilcox rank sum tests were used for the comparison of numerical variables between 2 groups; Pearson’s correlations were used for computing coefficients between imputed expression values. A p < 0.05 was considered as statistical significance