Fig. 3

hiPSC-derived lung progenitors engraft in an in vivo mouse model of airway injury. A: Schematic of the cell transplantation procedure. Mice were anesthetized and 30 µl of 2% Polydocanol was administered oropharyngeally. After 18 h, mice were anesthetised again and 30 µl of sterile PBS with 1% BSA and 1 million GFP + hiPSC-derived lung progenitors were administered to the back of the throat. Tracheas were harvested at different time points for analysis. B: Representative wholemount immunofluorescence staining showing GFP and DAPI at 7 days after transplantation (left) and GFP, human CK5 and DAPI at 10 days after transplantation (right). The scale bars are 50 μm