Fig. 6

NETs can activate ILK/β-parvin/RAC1 pathways to promote cytoskeletal remodeling and phenotypic transformation of PASMCs through CCDC25 in vitro. (A-B) Western blotting analysis of cytoskeletal related α-tubulin, β-tubulin, α-SMA, and SM22α in shCCDC25PASMCs. (C) Phalloidin staining to evaluated the effect of NETs on the cytoskeleton of shCCDC25 PASMCs. (D-E) Western blotting analysis of CCDC25, and ILK/β-parvin/RAC1 pathway in shCCDC25 PASMCs. Protein levels were normalized to β-actin. All results were presented as the mean ± SEM (n = 3). The p values were determined by Student’s t-test. NS: p > 0.05, * p < 0.05, ** p < 0.01, *** p < 0.001