Fig. 1
From: PI3Kγδ inhibition suppresses key disease features in a rat model of asthma
PI3Kγδ inhibitor, AZD8154, is able to inhibit both γ and δ isoforms of PI3K in rat cell-based assays. To determine if AZD8154 was able to inhibit rat PI3K isoforms rat blood neutrophils were pre-treated with AZD8154 and stimulated with CXCL1/CINC-1 to activate the PI3Kγ isoform (A); or rat splenic B cells were pre-treated with AZD8154 and stimulated with anti-IgM to activate the PI3Kδ isoform (B). Selective isoform inhibitors were included for reference and comparison (PI3Kγ inhibitor: AZD3458, and PI3Kδ inhibitor: GSK2269557). Data are presented as mean ± SEM. γ assay (A) & δ assay (B), pooled results from 3 independent experiments. Free IC50 (mean ± SEM) were calculated from n = 3 experiments by correcting for protein binding in the assays (A, B)