Figure 5

Administration of nebulized pDNA induces gene expression in the murine lung. Confocal microscopy of mouse lung parenchyma cryosections 24 hours after (a, b, c) dosage of 75 μg with post-nebulized pVR1020–YFP in 0.9% NaCl aqueous solution, compared to an (d, e, f) untreated case (scale bars: 100 μm). The lung structure (a, d) and (b, e) cell nuclei are indicated with a counterstain of hematoxylin and eosin, and with DAPI, respectively. Lung cells expressing YFP from instilled pVR1020–YFP appear green in (c) the treated lung sample; note the absence of green in the (f) untreated sample. The control lung samples were imaged at a higher resolution to confirm the absence of YFP response.